白细胞介素-1β(IL-1β)检测试剂盒(血清.血浆.组织)
产品简介
详细信息
白细胞介素-1β(IL-1β)检测试剂盒(血清.血浆.组织)英文名称Human Interleukin 1β,IL-1β ELISA Kit高品质elisa试剂盒,齐一生物科技有限公司提供的试剂盒受到了广大科研单位的*肯定和认同.*保证,价格公道,倾力为国内外科研院校实验室提供优质的产品.若有需要,我司将竭诚为您服务
类别:酶联免疫分析检测试剂盒
规格:96T/48T
包装:盒
检测原理:采用双抗夹心法
保质期:六个月
产品保存条件:2-8°C
应用范围:仅供科研研究实验使用
样品形式:血清/血浆/组织匀浆/细胞培养上清//其它生物液体(具体情况请咨询)
白细胞介素-1β(IL-1β)检测试剂盒标本的采集及保存:
1.血清:室温血液自然凝固10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清。保存 过程 中 如有沉淀形成,应再次离心。
2.血浆:应根据标本的要求选择EDTA、柠檬酸钠或肝素作为抗凝剂,混合10-20分钟后,离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
3.尿液:用无菌管收集。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。胸腹水、脑脊液参照此实行。
4.细胞培养上清:检测分泌性的成份时,用无菌管收集。离心20分钟左右(2000-3000转/ 分)。仔细收集上清。 检测细胞内的成份时,用PBS(PH7.2-7.4)稀释细胞悬液,细胞浓度达到100万/ml左右。通过反复冻融,以 使细胞破坏并 放出细胞内成份。离心20分钟左右(2000-3000转/分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。
5.组织标本:切割标本后,称取重量。加入一定量的PBS,PH7.4。用液氮迅速冷冻保存备用。标本融化后仍然保持2-8℃的温度。加入一定量的PBS(PH7.4),用手工或匀浆器将标本匀浆充分。离心20分钟左右(2000-3000转/分)。仔细收集上清。分装后一份待检测,其余冷冻备用。
标本要求:
1.标本采集后尽早进行提取,提取按相关文献进行,提取后应尽快进行实验。若不能马上进行试验,可将标本放于-20℃保存,但应避免反复冻融
2.不能检测含NaN3的样品,因NaN3抑制辣根过氧化物酶的(HRP)活性。
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胶体金标记一抗(多抗为抗原亲和纯化,单抗为G或A蛋白亲和纯化 )
GOLD*小鼠抗His Tag/Monoclonal Mouse Anti-His Tag*GOLD
GOLD*小鼠抗HBsAg/Monoclonal Mouse Anti-HBsAg*GOLD
GOLD*小鼠抗HBeAg/Monoclonal Mouse Anti-HBeAg*GOLD
GOLD*小鼠抗HBcAg/Monoclonal Mouse Anti-HBcAg*GOLD
GOLD*小鼠抗盐酸克伦特罗/Monoclonal Mouse Anti -Clenbuterol *GOLD
GOLD*小鼠抗三聚氰胺/Monoclonal Mouse Anti - Melamine *GOLD
GOLD*小鼠抗黄曲霉毒素B1/Monoclonal Mouse Anti - Aflatoxin B1 *GOLD
GOLD*小鼠抗赭曲霉素/Monoclonal Mouse Anti - ochratoxin *GOLD
GOLD*小鼠抗玉米赤霉烯酮/Monoclonal Mouse Anti - Zearalenone *GOLD
GOLD*小鼠抗伏马菌素/Monoclonal Mouse Anti - Fumonisin *GOLD
GOLD*兔抗*/Polyclonal Rabbit Anti - Salbutamol*GOLD
胶体金标记二抗(多抗为抗原亲和纯化,单抗为G或A蛋白亲和纯化)
GOLD*羊抗兔IgG (H+L) (兔IgG亲和纯化二抗标记)/Goat Anti-Rabbit IgG*GOLD
GOLD*羊抗小鼠IgG (H+L) (小鼠IgG亲和纯化二抗标记)/Goat Anti-Mouse IgG*GOLD
GOLD*小鼠抗人IgG (人 IgG亲和纯化二抗标记)/Monoclonal Mouse Anti—Human IgG*GOLD
GOLD*小鼠抗猴IgG(H+L) (猴 IgG亲和纯化二抗标记)/Monoclonal Mouse Anti---Monkey IgG*GOLD
GOLD*兔抗狗IgG (H+L) (狗IgG 亲和纯化二抗标记)/Rabbit Anti-Dog IgG *GOLD
GOLD*兔抗猪IgG (H+L)(猪 IgG亲和纯化二抗标记)/Rabbit Anti-Pig IgG *GOLD
GOLD*兔抗鸡IgY IgG(H+L) (鸡 IgG亲和纯化二抗标记)/Rabbit Anti-Chicken *GOLD
GOLD*兔抗马IgG (H+L) (马 IgG亲和纯化二抗标记)/Rabbit Anti-Horse *GOLD
GOLD*兔抗山羊IgG (H+L) (山羊 IgG亲和纯化二抗标记)/Rabbit Anti-Goat IgG *GOLD
白细胞介素-1β(IL-1β)检测试剂盒GOLD*兔抗人IgG (FC) (人 IgG(FC)亲和纯化二抗标记) /Rabbit Anti-Human IgG(FC) *GOLD
GOLD*兔抗猴IgG (H+L) (猴 IgG亲和纯化二抗标记)/Rabbit Anti-monkey IgG *GOLD
GOLD*兔抗牛IgG(H+L) ( 牛 IgG亲和纯化二抗标记)/Rabbit Anti-Bovine IgG *GOLD
GOLD*兔抗豚鼠IgG(H+L) (豚鼠 IgG亲和纯化二抗标记)/Rabbit Anti-Guinea pig IgG *GOLD
GOLD*兔抗齐一生物供应IgG (H+L) (齐一生物供应 IgG亲和纯化二抗标记)/Rabbit Anti-Rat IgG *GOLD
GOLD*兔抗鸭IgY IgG (H+L)鸭 IgG亲和纯化二抗标记/Rabbit Anti-Duck IgY*GOLD
GOLD*兔抗猫IgG (H+L) (猫IgG亲和纯化二抗标记)/Rabbit Anti-Cat IgG *GOLD
GOLD*小鼠抗猪IgG (H+L) (猪 IgG亲和纯化二抗标记)/Monoclonal Mouse Anti—pig IgG*GOLD
GOLD*兔抗绵羊IgG (H+L) (绵羊IgG亲和纯化二抗标记)/Rabbit Anti-Sheep IgG *GOLD
小分子抗原
牛血清白蛋白偶联克伦特罗
鸡卵清蛋白偶联克伦特罗
牛血清白蛋白偶联三聚氰胺
鸡卵清蛋白偶联三聚氰胺
牛血清白蛋白偶联*
基因重组抗原
人类丙型肝炎病毒重组抗原/ HCV
人类乙型肝炎病毒重组E抗原 /HBeAg