1.50436.0001 Merck 密理博 HPLC色谱柱
产品简介
详细信息
Merck 密理博 HPLC色谱柱
详细介绍
Merck ZIC-HILIC 亲水作用色谱柱
● 直接分离极性和亲水性化合物
● 两性离子基团通过共价键合在多孔硅胶上
● 超长的耐受性使得样品分离的成本很低
● ZIC-pHILIC,聚合物基质,PH2-10
为什么使用HILIC?
尽管反相液相色谱(RPLC)是至今应用非常广泛的色谱分离技术,它能与各种常规的检测方法结合,解决多种分析应用问题,但对某些分析物,特别是极性和亲水性化合物却无法或很少保留。长期以来,人们采用正相液相色谱(NPLC),并使用不利环保的非水溶性流动相如己脘或环己脘来分析这些物质。
但是在这种实验条件下,很多极性和亲水化合物往往很难溶解,从而限制了NPLC的应用范围。从现在起有了另一种新的解决办法,即选用亲水作用液相色谱-HILIC。
什么是亲水作用色谱(HILIC):
亲水作用液色谱(Hydrophilic Interaction LiquidChromatography,HILIC) 是一种直接分离极性和亲水性化合物的色谱技术,为了便于理解,我们可以把它看作为是一种反相色谱溶剂做为流动相的正相色谱。
因此HILIC是:
● 亲水性的固定相
● 以水、有机溶剂和缓冲液作为流动相洗脱剂
常见的HILIC方法采用50-90%有机溶剂和一定比例的水,并且含有低浓度的缓冲盐。如乙腈/水/醋酸铵体系
在HILIC方法中,洗脱顺序和反相色谱正好相反,即极性越大,保留时间越长。
Merck 密理博 HPLC色谱柱
订货信息:
货号 描述 固定相 规格
1.50434.0001 ZIC®-HILIC (5µm) 14 x 1 mm PEEK保护柱(5件) fitting guard column
1.50435.0001 ZIC®-HILIC (5µm) 20 x 2.1 mm PEEK保护柱 (1件) fitting guard column
1.50436.0001 ZIC®-HILIC (5µm) 20 x 2.1 mm PEEK保护柱套件 (3件) fitting guard column
1.50439.0001 ZIC®-HILIC (3.5µm, 100Å) 20 x 2.1 mm PEEK HPLC column
1.50440.0001 ZIC®-HILIC (3.5µm, 100Å) 50 x 2.1 mm PEEK HPLC column
1.50441.0001 ZIC®-HILIC (3.5µm, 100Å) 100 x 2.1 mm PEEK HPLC column
1.50442.0001 ZIC®-HILIC (3.5µm, 100Å) 150 x 2.1 mm PEEK HPLC column
1.50444.0001 ZIC®-HILIC (3.5µm, 100Å) 150 x 4.6 mm PEEK HPLC column
1.50443.0001 ZIC®-HILIC (3.5µm, 100Å) 250 x 2.1 mm PEEK HPLC column
1.50445.0001 ZIC®-HILIC (3.5µm, 200Å) 50 x 2.1 mm PEEK HPLC column
1.50446.0001 ZIC®-HILIC (3.5µm, 200Å) 50 x 4.6 mm PEEK HPLC column
1.50447.0001 ZIC®-HILIC (3.5µm, 200Å) 100 x 2.1 mm PEEK HPLC column
1.50448.0001 ZIC®-HILIC (3.5µm, 200Å) 150 x 2.1 mm PEEK HPLC column
1.50449.0001 ZIC®-HILIC (3.5µm, 200Å) 150 x 4.6 mm PEEK HPLC column
1.50450.0001 ZIC®-HILIC (5µm, 200Å) 50 x 2.1 mm PEEK HPLC column
1.50451.0001 ZIC®-HILIC (5µm, 200Å) 50 x 4.6 mm PEEK HPLC column
1.50452.0001 ZIC®-HILIC (5µm, 200Å) 100 x 2.1 mm PEEK HPLC column
1.50453.0001 ZIC®-HILIC (5µm, 200Å) 100 x 4.6 mm PEEK HPLC column
1.50454.0001 ZIC®-HILIC (5µm, 200Å) 150 x 2.1 mm PEEK HPLC column
1.50455.0001 ZIC®-HILIC (5µm, 200Å) 150 x 4.6 mm PEEK HPLC column
1.50456.0001 ZIC®HILIC (5µm, 200Å) 150 x 7.5 mm PEEK HPLC column
1.50457.0001 ZIC®-HILIC (5µm, 200Å) 250 x 2.1 mm PEEK HPLC column
1.50458.0001 ZIC®-HILIC (5µm, 200Å) 250 x 4.6 mm PEEK HPLC column
1.50437.0001 ZIC®-pHILIC polymeric (5µm) 20 x 2.1 mm PEEK Guard (1 pc) PEEK保护柱(1件)
1.50438.0001 ZIC®-pHILIC polymeric (5µm) 20 x 2.1 mm PEEK Guard Kit PEEK保护柱套件(3件)
1.50462.0001 ZIC®-pHILIC polymeric (5µm) 100 x 2.1 mm PEEK metal-free HPLC column
1.50460.0001 ZIC®-pHILIC polymeric (5µm) 150 x 2.1 mm PEEK metal-free HPLC column
1.50464.0001 ZIC®-pHILIC polymeric (5µm) 100 x 4.6 mm PEEK metal-free HPLC column
1.50463.0001 ZIC®-pHILIC polymeric (5µm) 50 x 4.6 mm PEEK metal-free HPLC column
1.50459.0001 ZIC®-pHILIC polymeric (5µm) 50 x 2.1 mm PEEK metal-free HPLC column
1.50461.0001 ZIC®-pHILIC polymeric (5µm) 150 x 4.6 mm PEEK metal-free HPLC column