实验型超滤系统LDS-L1

Experimental Ultrafiltration System LDS-L1

切向流过滤

Tangential Flow Filtration，TFF

切向流过滤（Tangential Flow Filtration，TFF）是目前应用非常广泛的一种膜分离方法。样品在压力驱动下，内容物根据分子尺寸不同实现膜过滤分离，达到去除热原、置换溶液环境、纯化和浓缩等目的。

Tangential Flow Filtration (TFF) is a widely used membrane separation method. Driven by pressure, the contents of the sample can be separated by membrane filtration according to different molecular sizes, so as to remove pyrogen, replace solution environment, purify and concentrate.

切向流过滤的膜部件通常是膜包或者中空纤维柱，二者均有多种型号，以满足不同的使用需求。膜材种类和孔径大小、溶液循环流速、膜两侧压力、样品温度、工艺时间等参数，是切向流过滤非常重要的参数，它们决定了最终的工艺效果，以及大规模生产的可行性及具体方案。实验室规模的切向流过滤设备致力于获得可行的工艺方案和可靠的工艺参数，为大规模试验和生产确定适合的工艺参数，提供设备或系统选型的有效依据。

The membrane components of tangential flow filtration are usually membrane package or hollow fiber column, both of which have a variety of models to meet different use needs. Membrane material type and pore size, solution circulation flow rate, pressure on both sides of the membrane, sample temperature, process time and other parameters are very important parameters for tangential flow filtration. They determine the final process effect, as well as the feasibility and specific scheme of large-scale production. The laboratory scale tangential flow filtration equipment is committed to obtaining feasible process schemes and reliable process parameters, determining appropriate process parameters for large-scale test and production, and providing an effective basis for equipment or system selection.

包裹小分子药物的脂质体和包裹核酸药物的脂质纳米颗粒是当下非常热门的两类药物载体，他们在研发和生产中均需使用切向流过滤处理样品。

Liposomes coated with small molecule drugs and lipid nanoparticles coated with nucleic acid drugs are two very popular drug carriers. They need to use tangential flow filtration to treat samples in R & D and production.

图1：超滤系统流程图

Figure 1: flow chart of ultrafiltration system

从滤膜的截留孔径进行分类，可以将过滤分为澄清过滤、微滤、超滤、纳滤和反渗透等。将不同孔径的滤膜采用切向流的操作方式进行过滤，即可称之为切向流微滤和切向流纳滤等。

The filtration can be divided into clarification filtration, microfiltration, ultrafiltration, nanofiltration and reverse osmosis. Tangential flow microfiltration and tangential flow nanofiltration can be referred to as tangential flow microfiltration and tangential flow nanofiltration.

图2：滤膜孔径分类

Figure 2: filter membrane pore size classification

艾特森LDS系列超滤系统功能

Functions Of AITESEN LDS Series Ultrafiltration System

该系统主要应用于样品的浓缩、提纯、透析、置换缓冲液和溶液除热原等需求领域。过程中可通过无纸记录仪或液晶面板显示及记录样品的重量、过膜前压力、回流压力、透过压力、跨膜压TMP等数据。

The system is mainly used in the demａnd fields of sample concentration, purification, dialysis, replacement buffer and solution pyrogen removal. In the process, the weight, pre membrane pressure, reflux pressure, permeation pressure, transmembrane pressure TMP and other data of the sample can be displayed and recorded through paperless recorder or liquid crystal panel.

应用领域包括：

Applications include:

Ÿ   化学药品脂质体（Liposome）纯化，如盐酸脂质体、伊立替康脂质体等；

Purification of chemical drug Liposomes, such as doxorubicin hydrochloride liposomes and irinotecan liposomes;

Ÿ   核酸药物和疫苗纯化，如脂质纳米颗粒、聚合物纳米颗粒、mRNA-LNP、siRNA-LNP等；

Purification of nucleic acid drugs and vaccines, such as lipid nanoparticles, polymer        nanoparticles, mRNA LNP, siRNA LNP, etc;

Ÿ   生物细胞类产品分离和纯化，可从发酵液中收集细胞并清除细胞碎片等；

Separation and purification of biological cell products, which can collect cells from        fermentation broth and remove cell fragments;

Ÿ   疫苗、单抗和重组蛋白药物的纯化；

Purification of vaccines, monoclonal antibodies and recombinant protein drugs;

Ÿ   置换缓冲溶液；

Displacement buffer solution;

Ÿ   基于分子大小原理分离和纯化的其他用途。

Other uses of separation and purification based on molecular size principle.

技术参数

Technical Parameter

艾特森可根据用户需求进行定制型设计和集成，其技术参数另行提供。可定制类型包括：

AITESEN can carry out customized design and integration according to user needs, and its technical parameters are provided separately. Customizable types include:

Ÿ   不同样品体积；

  Different sample volumes;

Ÿ   不同控制方式的要求；

  Requirements of different control modes;

Ÿ   不同配制档次的要求；

  Requirements of different preparation grades;

Ÿ   通用/专用型要求；

         General / special requirements;