详细介绍

超高性能通用的丙基氰基键合相，提供*的峰形，增加保留，在低PH值条件下具有延长柱寿命。高强度硅胶颗粒（HSS）技术专为补充杂化BEH和CSH颗粒开发，用于提高色谱效率。高孔径容量的硅胶基质HPLC颗粒不具备必要的机械稳定性来承受UPLC分离过程中的压力，如果实验室需要将现有的HPLC方法转移到UPLC系统上的话，这一点就显得尤为重要。机械稳定性的限制促使沃特世的科学家设计出一种颗粒具有高机械稳定性和较长的使用寿命以及高压下的UPLC的柱效。

与直链烷烃色谱柱相比，ACQUITY UPLC HSS氰基色谱柱提供低疏水性和选择性。由于采用创新的氰基单点键合配以保护性烷基侧链，ACQUITY UPLC HSS氰色谱柱在低至中等pH条件下具有超稳定的保留性能、优异峰形和可重现性。另外HSS氰色谱柱同时兼容于反相和正相技术。

Simultaneous determination of etonogestrel and ethinyl estradiol in human plasma by UPLC-MS/MS and its pharmacokinetic study
A selective, sensitive and rapid ultra-performance liquid chromatography tandem mass spectrometry method was developed and validated for the simultaneous determination of etonogestrel (ENG) and ethinyl estradiol (EE) in human plasma. The analytes and their deuterated internal standards, ENG-d7 and EE-d4, were extracted from plasma samples by solid-phase extraction on HyperSep™ Retain PEP cartridges. The chromatographic analysis was performed on an Acquity UPLC HSS Cyano column, 100 Å (50 × 2.1 mm, 1.8 μm), column using gradient phase, acetonitrile and 2.0 mm ammonium trifluoroacetate at 0-1.7 min (65:35, v/v) and 1.8-2.7 min (95:5, v/v) with 0.250 mL/min flow rate. Analytes and IS protonated precursor → product ion transitions (ENG, m/z 325.2 → 257.2; EE, m/z 530.2 → 171.2; ENG-d7, m/z 332.2 → 263.2; EE-d4, m/z 534.2 → 171.2) were monitored on a Triple Quadrupole Mass spectrometer (TQMS), operating in multiple reaction monitoring and positive ionization mode. The calibration curves were established at 10.00-2500 pg/mL for ENG and 1.500-150.0 pg/mL for EE with a correlation coefficient (r2 ) ≥0.9996 for both. The validated method was successfully applied to support a bioequivalence study of 0.15 mg ENG and EE 0.03 mg tablet formulation, administered in 24 healthy Indian females. Method reliability was assessed by reanalysis of 94 incurred study samples.

品名	编号
ACQUITY - Xselect HSS Cyano (CN) Method Transfer Kit, 100Å, 1.8 - 3.5 µm, 2.1 - 4.6 mm X 100 - 15...	186006002
ACQUITY - Xselect HSS Cyano (CN) 100Å, 1.8 - 3.5 µm, 2.1 - 4.6 mm X 50 - 100mm, 2/pkg	186006001
ACQUITY - Xselect HSS Cyano (CN) Method Transfer Kit, 100Å, 1.8 - 5 µm, 2.1 - 4.6 mm X 50 - 150mm...	186006000
ACQUITY UPLC HSS Cyano (CN) Method Validation Kit, 100Å, 1.8 µm, 2.1 mm X 50 mm, 3/pkg	186005996
ACQUITY UPLC HSS Cyano (CN) Method Validation Kit, 100Å, 1.8 µm, 2.1 mm X 100 mm, 3/pkg	186005997
ACQUITY UPLC HSS Cyano (CN) Column, 100Å, 1.8 µm, 3 mm X 100 mm, 1/pkg	186005993
ACQUITY UPLC HSS Cyano (CN) Column, 100Å, 1.8 µm, 3 mm X 75 mm, 1/pkg	186005992
ACQUITY UPLC HSS Cyano (CN) Column, 100Å, 1.8 µm, 3 mm X 150 mm, 1/pkg	186005994
ACQUITY UPLC HSS Cyano (CN) Column, 100Å, 1.8 µm, 3 mm X 50 mm, 1/pkg	186005991
ACQUITY UPLC HSS Cyano (CN) Column, 100Å, 1.8 µm, 3 mm X 30 mm, 1/pkg	186005990
ACQUITY UPLC HSS Cyano (CN) Column, 100Å, 1.8 µm, 2.1 mm X 150 mm, 1/pkg	186005989
ACQUITY UPLC HSS Cyano (CN) Column, 100Å, 1.8 µm, 2.1 mm X 75 mm, 1/pkg	186005987
ACQUITY UPLC HSS Cyano (CN) Column, 100Å, 1.8 µm, 2.1 mm X 100 mm, 1/pkg	186005988
ACQUITY UPLC HSS Cyano (CN) Column, 100Å, 1.8 µm, 2.1 mm X 30 mm, 1/pkg	186005985
ACQUITY UPLC HSS Cyano (CN) Column, 100Å, 1.8 µm, 2.1 mm X 50 mm, 1/pkg	186005986
ACQUITY UPLC HSS Cyano (CN) Column, 100Å, 1.8 µm, 2.1 mm X 100 mm, 3/pkg	176002709
ACQUITY UPLC HSS Cyano (CN) Column, 100Å, 1.8 µm, 1 mm X 150 mm, 1/pkg	186005984
ACQUITY UPLC HSS Cyano (CN) Column, 100Å, 1.8 µm, 1 mm X 100 mm, 1/pkg	186005983
ACQUITY UPLC HSS Cyano (CN) Column, 100Å, 1.8 µm, 1 mm X 50 mm, 1/pkg	186005982
ACQUITY UPLC HSS Cyano (CN) Method Validation Kit, 100Å, 1.8 µm, 3 mm X 50 mm, 3/pkg	186005998
ACQUITY UPLC HSS Cyano (CN) Method Validation Kit, 100Å, 1.8 µm, 3 mm X 100 mm, 3/pkg	186005999
ACQUITY UPLC HSS Cyano (CN) VanGuard Pre-column, 100Å, 1.8 µm, 2.1 mm X 5 mm, 3/pkg	186005995

产品简介

详细介绍