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ACQUITY UPLC CSH C18 Column
面议
ACQUITY UPLC CSH Phenyl-Hexyl Column
面议
ACQUITY UPLC HSS T3 Column
面议
ACQUITY UPLC BEH HILIC柱
面议
ACQUITY UPLC BEH Shield RP18 Column
面议
ACQUITY UPLC BEH Phenyl Column
面议
ACQUITY UPLC BEH Amide Column
面议
ACQUITY UPLC HSS Cyano Column
面议
ACQUITY UPLC HSS C18 （SB）Column
面议
ACQUITY UPLC系统
面议
Alliance HPLC系统
面议
Breeze QS HPLC系统
面议五氟苯基(PFP：Pentafluorophenyl，或又称全氟苯基)配体提供多重保留机制包括：氢键作用、偶极-偶极作用、芳香性(π-π)作用，以及疏水(反相)作用，当试图分离异构性的、芳香性的、或是卤代的化合物特别有用。
含氟固定相为常规C18提供了互补的色谱分离选择性，特别是当需要分析碱性或平面芳香族化合物时。XSelectHSS PFP和CSH氟苯基色谱柱具有相同配体，但是不同的颗粒基体提供了不同的保留特性。
ACQUITY UPLC HSS PFP色谱柱为三键键合、非封端的五氟苯基(PFP)化学特性，与市场上其他PFP固定相相比，可提供行业的可重现性和优异峰形。由于综合了多种保留机理，包括芳香(π-π)作用、氢键作用、偶极-偶极作用和疏水作用等,ACQUITY UPLC HSS PFP色谱柱理想用于平面芳香族化合物、位置异构体、卤代化合物以及极性分析物。
目的建立同时测定人血浆中,,,及其代谢物浓度的方法.方法采用超高效液相色谱-质谱联用法(UPLC-MS/MS),以(SMZ)为内标,血浆经甲醇直接沉淀后进样分析.色谱柱为Waters ACQUITY UPLC HSS PFP柱(2.1 mm×100 mm,1.8μm),流动相为0.1%甲酸的5 mmol·L-1乙酸铵水溶液-0.1%甲酸的甲醇溶液(0~5 min,35:65→10:90),流速为0.2 mL·min-1.电喷雾离子源,正离子多反应监测扫描分析,,,,和的离子对分别为m/z 237.0→194.06,m/z 255.98→144.95,m/z 316.01→270.0,m/z 285.04→193.07和m/z 287.02→241;内标的离子对为m/z 253.96→91.97.结果,,,和血药浓度分别在2.4~600 ng·mL-1(r=0.999 7),2.52~630 ng·mL-1(r=0.992 0),2.08~520 ng·mL-1(r=0.997 9),2.28~570 ng·mL-1(r=0.998 2),8.0~800 ng·mL-1(r=0.999 2)线性关系良好;检出限分别为0.24,0.63,0.52,0.57,3.2 ng·mL-1.日内,日间精密度均15%;提取回收率均70%,且RSD15%.结论该方法灵敏,快速,专属性强,可用于临床血药浓度测定及药动学研究.
品名 | 编号 |
ACQUITY - Xselect HSS PFP Method Transfer Kit, 100Å, 1.7 - 3.5 µm, 2.1 - 4.6 mm X 50 - 100mm, 1/pk... | 186005980 |
ACQUITY - Xselect HSS PFP Method Transfer Kit, 100Å, 1.8 - 3.5 µm, 2.1 - 4.6 mm X 100 - 150mm, 1/p... | 186005981 |
ACQUITY UPLC HSS PFP VanGuard Pre-column, 100Å, 1.8 µm, 2.1 mm X 5 mm, 3/pkg | 186005974 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 3 mm X 75 mm, 1/pkg | 186005971 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 3 mm X 50 mm, 1/pkg | 186005970 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 3 mm X 150 mm, 1/pkg | 186005973 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 3 mm X 100 mm, 1/pkg | 186005972 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 2.1 mm X 50 mm, 1/pkg | 186005965 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 2.1 mm X 75 mm, 1/pkg | 186005966 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 1 mm X 150 mm, 1/pkg | 186005963 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 2.1 mm X 30 mm, 1/pkg | 186005964 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 3 mm X 30 mm, 1/pkg | 186005969 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 2.1 mm X 100 mm, 1/pkg | 186005967 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 2.1 mm X 150 mm, 1/pkg | 186005968 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 1 mm X 100 mm, 1/pkg | 186005962 |
ACQUITY UPLC HSS PFP Column, 100Å, 1.8 µm, 1 mm X 50 mm, 1/pkg | 186005961 |
